NEB

New England Biolabs — enzymes and reagents for life science research

About NEB

New England Biolabs is now a recognized world leader in the discovery and production of enzymes for molecular biology applications. NEB offers the largest selection of recombinant and native enzymes for genomic research. While restriction enzymes remain part of their core product portfolio, their ever-expanding catalog also includes products related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, and Glycobiology, epigenetics and RNA analysis.

Illume Gene Bio-Freezer Program

@ Indian Institute of Science (IISc) — Biostores

Illume Gene Bio-Freezer Program at IISc Biostores — NEB reagents flyer with product highlights including T4 DNA Ligase, Q5 Hot Start Master Mix, and 100 bp DNA Ladder

Program Flyer

Product Categories

DNA restriction enzyme recognition site diagram showing TGCA sequence with cut positions marked by triangles on both strands

Restriction enzyme recognition & cleavage site

Restriction Endonucleases

Restriction Endonucleases: A
Restriction Endonucleases: B
Restriction Endonucleases: C-G
Restriction Endonucleases: H-M
Restriction Endonucleases: N-O
Restriction Endonucleases: P-R
Restriction Endonucleases: S
Restriction Endonucleases: T-Z
HF®, Nicking, Master Mix, Time-Saver & Other
Restriction Endonuclease Buffers & Diluents

Q5 High-Fidelity DNA Polymerase vials from New England BioLabs — Q5 High-Fidelity, Q5 Hot Start, and Q5 Reaction Buffer

PCR, QPCR & Amplification Technologies

DNA Polymerases

Q5® High-Fidelity DNA Polymerases
OneTaq® DNA Polymerases
Taq DNA Polymerase
LongAmp® DNA Polymerases
Deep Vent® (exo-) DNA Polymerase
Deep Vent® DNA Polymerase
Hemo KlenTaq®
Vent® (exo-) DNA Polymerase
Vent® DNA Polymerase
Phusion™ High-Fidelity DNA Polymerases

Q5 High-Fidelity 2X Master Mix tubes from New England BioLabs — product code M0492S

Mastermixes

Hot Start Taq 2X Master Mix
LongAmp® Hot Start Taq 2X Master Mix
LongAmp® Taq 2X Master Mix
Multiplex PCR 5X Master Mix
NEBNext® Q5® Hot Start HiFi PCR Master Mix
OneTaq® 2X Master Mix with Standard Buffer
OneTaq® Hot Start 2X Master Mix with GC Buffer
OneTaq® Hot Start 2X Master Mix with Standard Buffer
OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer
OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
Phusion™ High-Fidelity PCR Master Mix with GC Buffer
Phusion™ High-Fidelity PCR Master Mix with HF Buffer
Phusion™ Hot Start Flex 2X Master Mix
Q5® High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Blood Direct 2X Master Mix
Quick-Load® Taq 2X Master Mix
Taq 2X Master Mix
Taq 5X Master Mix
WarmStart® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

New England BioLabs Quick Blunting Kit and dNTP reagent tubes — blunting enzyme, buffer, and dNTPs for DNA end repair

DNA Manipulation

Bsu DNA Polymerase, Large Fragment
DNA Polymerase I (E. coli)
DNA Polymerase I, Large (Klenow) Fragment
Klenow Fragment (3´→5´ exo-)
PreCR® Repair Mix
Quick Blunting™ Kit
Sulfolobus DNA Polymerase IV
T4 DNA Polymerase
T7 DNA Polymerase (unmodified)
Terminal Transferase

LunaScript Multiplex One-Step RT-PCR Reaction Mix tubes from New England BioLabs — M1556S, M1557S, and Nuclease-free Water B1502A

Kits for RT-PCR, qPCR & RT-qPCR & Other Reagents

Control LAMP Primer Mix (rActin)
dGTP Solution
dUTP Solution
Luna® qPCR & RT-qPCR Kits
Isothermal Amplification & Strand Displacement
Nucleotide Solutions
cDNA Synthesis & Reverse Transcriptases
RT-PCR Kits
Reaction Buffers

T4 DNA Ligase substrate diagram showing dsDNA and Nicked DNA/RNA ligation substrates with 5' OH and 3' P strand representations

DNA Modifying Enzymes

Argonautes
DNA Ligase
DNA Repair Enzymes and Structure-specific Endonucleases
Methyltransferase
Exonucleases and Non-specific Endonucleases
Phosphatase
Recombinase
ssDNA Binding Protein
Kinase
DNA Labeling

NEBNext NGS library preparation workflow diagram showing End Repair, Adaptor Ligation, PCR Enrichment, Clean Up steps for Illumina and FFPE DNA inputs

Next Generation Sequencing Library Preparation

Library Preparation for Illumina
Library Preparation for Ion Torrent
Library Preparation for Oxford Nanopore Technologies
Infectious Diseases & ARTIC Sequencing
DNA Fragmentation & RNA Fragmentation
RNA Depletion & mRNA Enrichment
Methylome Analysis
Microbiome DNA Enrichment
NEBNext® Multiplex Oligos (Adaptors & Primers)
FFPE DNA
Library Preparation for MGI®
NGS Library Quantitation
Automation for NEBNext® NGS Library Prep
Small RNA Library Preparation

4-step nucleic acid purification workflow diagram — Dissolve, Bind, Wash, Elute — showing column-based DNA extraction from gel slice to high-quality purified DNA

Nucleic Acid Purification

Plasmid Purification
PCR & DNA Cleanup
DNA Gel Extraction
Genomic DNA Extraction & Purification
High Molecular Weight DNA Extraction
Total RNA Extraction & Purification
RNA Cleanup
Magnetic Bead-Based Nucleic Acid Purification

DNA ladder gel electrophoresis image showing molecular weight markers ranging from 0.1 to 10.0 kilobases with mass values in nanograms

Markers & Ladders

Quick-Load® Purple 1 kb Plus DNA Ladder
1 kb Plus DNA Ladder
1 kb Plus DNA Ladder for Safe Stains
Quick-Load® Purple 100 bp DNA Ladder
100 bp DNA Ladder
Quick-Load® Purple 1 kb DNA Ladder
1 kb DNA Ladder
Protein Standards
Quick-Load Purple DNA Ladders
RNA Markers & Ladders
Gel Loading Buffers

Monarch RNA Lysis Buffer bottles from New England BioLabs — Part T2012-1, 50 ml, for RNA extraction and purification

RNA Synthesis and Modification

RNA Capping
RNA Extraction and Purification
RNA Ligation
RNA Markers and Ladders
RNA Modification
RNA Synthesis In vitro Transcription (IVT)
RNase Inhibitors
RNases

Q5 Site-Directed Mutagenesis Kit Quick Protocol diagram showing three steps: PCR amplification, KLD treatment, and high-efficiency transformation

DNA Assembly, Cloning and Mutagenesis Kits

Digestion Protocol for BioBrick Assembly Kit
Ligation Protocol using BioBrick Assembly Kit®
Gibson Assembly® Protocol
Protocol for Control Reaction
Quick Protocol for Q5® Site-Directed Mutagenesis Kit
Gibson Assembly® Chemical Transformation Protocol
Gibson Assembly® Electrocompetent Transformation Protocol
Insert Screening Protocols for NEB PCR Cloning Kit
Ligation Protocol for NEB PCR Cloning Kit
Plating Protocol for NEB PCR Cloning Kit
Transformation Protocol for NEB PCR Cloning Kit
Quick Protocol for Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)
NEBuilder® HiFi DNA Assembly Electrocompetent Transformation Protocol
NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol

SNAP-tag and CLIP-tag protein labeling mechanism diagram showing fusion protein binding with benzylguanine and benzylcytosine chemical labels

Cellular Analysis

SNAP-tag Substrates
CLIP-tag™ Substrates
SNAP-Capture
Blocking Agents
Cellular Analysis Vectors
Biotin Labels
Building Blocks
SNAP-tag Other

Other Kits & Reagents

Epigenetics

Chromatin Analysis
Epitranscriptome Analysis
DNA Methylation Analysis
Methylome Analysis
Methyltransferases for Epigenetics

Protein Expression & Purification

E. coli Protein Expression Strains
Cell-Free Protein Expression
Bacterial E. coli Protein Expression
Protein Expression in Yeast
Affinity Purification
Magnetic Beads and Racks

Competent Cells

Cloning Competent Cell Strains
E. coli Expression Strains
Yeast Expression Strains
Competent Cells Buffers & Diluents

DNA Plasmids & Substrates

Lambda DNA
Lambda DNA (dam-)
M13mp18 RF I DNA
M13mp18 Single-stranded DNA
pBR322 Vector
pCLIPf Vector
pMAL-c6T Vector
pSNAPf Vector
pSNAP-tag® (T7)-2 Vector
pTWIN1 Vector
pTXB1 Vector
pTYB21 Vector
pUC19 Vector
ΦX174 RF I DNA
ΦX174 RF II DNA
ΦX174 Virion DNA

Protein Tools

Products coming soon — contact us for availability.

Glycobiology

Products coming soon — contact us for availability.

Buffers

Products coming soon — contact us for availability.

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