Illume Gene India LLP

MGI Stomics — Illume Gene India LLP

MGI STOMICS

Spatial transcriptomics for single-cell resolution tissue analysis

About MGI STOMICS

STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide V1.3 is intended for generating a spatially-resolved 3′ mRNA library from biological tissue sections. This enables a "tissue-to-data" solution through in situ capture of the whole transcriptome at nanoscale resolution and centimeter-sized Field of View (FOV). This kit uses DNB-patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ.

Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.

Stomic Solutions

STOmics Solution Stereo-seq Transcriptomics

  • •This enables a "tissue-to-data" experience through in situ capturing of the whole transcriptome at nanoscale resolution and centimeter-sized field of view.

Product Highlights

  • •Compatible with variety of tissue types & species as well as precious samples with low RIN values
  • •Improved capture efficiency giving higher gene counts per cell
STOmics Stereo-seq transcriptome tissue visualization

Stereo-seq Large Chip Designs

  • •This achieves both centimeter level FOV (now up to 2cm x 3cm) with subcellular resolution for efficient capturing of the whole transcriptome with true nanoscale resolution.

Product Highlights

  • •Compatible for all species
  • •Large capture area without compromising the capture efficiency
  • •Proprietary cell segmentation algorithm integrated on Stereo-seq Analysis Workflow converting data to information
Stereo-seq large chip designs showing centimeter-level FOV tissue scans

Stereo-cite Proteo-Transcriptomics Solution

  • •This explores the study of tissue heterogeneity and associated biological significance in disease tissues, leading to a broader understanding of spatial omics studies with true single-cell resolution for both transcriptome & protein analysis.

Product Highlights

  • •Simultaneous detection of the whole transcriptome and high detection efficiency of 100+ plex protein antibody combinations of your choice on the same tissue section.
  • •No auto-fluorescence interference or antigen instability caused by multiple rounds of detection.
Stereo-cite proteo-transcriptomics multiplexed protein and whole-transcriptome spatial analysis

Stereo-seq Omni for FFPE Solution

  • •This spatial transcriptomics solution helps to study your FFPE samples. It provides true spatial single-cell total RNA information with accuracy and precision with the innovative 'Random Probe' design.

Product Highlights

  • •Compatible with low-quality samples
  • •True single-cell level gene expression profiling combined with histology study
  • •Species agnostic, enabling study of host interaction with spatial context
  • •Random probe design for efficient total RNA capturing
Stereo-seq Omni FFPE spatial transcriptomics multi-layer tissue RNA analysis

Stereo-seq Transcriptomics Set for MIF

  • •This allows for in-depth spatial multi-omics discovery & also enables spatial visualization of multiple proteins in single-cell resolution on top of the unbiased whole transcriptome information on the same tissue section.

Product Highlights

  • •High fidelity protein staining imaging for enhanced spatial protein detection
  • •Detect multiple proteins with unbiased whole transcriptomics & flexibility in choosing antibodies based on user's need
  • •Co-detection of protein and RNA in a single experiment for extensible applications in tissue and cellular type study
Stereo-seq MIF spatial multi-omics fluorescent tissue imaging

Stomics Products & Workflow

STOmics Products

Overall Components:

  • •Stereo-seq Permeabilization Kit
  • •Stereo-seq Chip P (1cm × 1cm) (1cm × 2cm), (2cm × 2cm), (2cm × 3cm)
  • •STOmics Accessory Kit
  • •Stereo-seq Transcriptomics T Kit
  • •Stereo-seq Chip T Slide (1cm × 1cm)
  • •Stereo-seq Chip N Slide (1cm × 1cm)
  • •Stereo-seq Protein Assisted Kit
  • •Stereo-seq16 Barcode Library Preparation Kit V1.0
  • •Stereo-seq PCR Adaptor
  • •STOmics FFPE Accessory Kit
  • •Stereo-seq Transcriptomics N Kit
  • •Stereo-seq Library Preparation Kit
STOmics product kits including Stereo-seq Permeabilization Kit, Chip P Slide, Library Preparation Kit, Chip T Slide and Transcriptomics Kit

Protocol

  1. 1Sectioning & mounting of fresh frozen samples on Stereo-seq Chip T Slide
  2. 2Stain the tissue with nuclei- or hematoxylin and eosin (H&E) stain
  3. 3Visualize it using an epi-fluorescence microscope or reflected brightfield microscope
  4. 4Tissue permeabilization and mRNA release
  5. 5Released mRNA is captured by spatially barcoded probes on Stereo-seq Chips
  6. 6Sequence on Stereo-seq Transcriptome FF library on DNBSEQ-G400 or DNBSEQ-T7
  7. 7Stereo-seq Analysis Workflow (SAW) to process your spatial gene expression data
Stereo-seq Analysis Workflow diagram showing six sequential steps: Sample preparation, Imaging, mRNA capture and cDNA synthesis, Library construction, Sequencing, and Data Analysis and Visualization

Application

STOmics spatial biology layered imaging showing multicolor heat maps of tissue sections at tissue, cellular, subcellular and molecular resolution

This novel tool explores spatial biology with unprecedented field-of-view, and subcellular resolution, enabling simultaneous transcriptome study and analysis at tissue-, cellular-, subcellular- and molecular level.

Compatible with fresh frozen samples, and has reliable performance on cells, organs, and embryo sections of various animal and plant species.

Visit MGI STOMICS Website

Illume Gene India LLP

#981, Gokula, 3rd Floor, 18th cross Near,
Ideal Homes Township,
Rajarajeshwari Nagar,
Bengaluru, Karnataka 560098

[email protected]

Mon–Sat: 09:30–18:30 | Sunday: Closed

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