STOmics (SpaTial Enhanced REsolution Omics)
BRIEF OVERVIEW
- STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide V1.3 is intended for generating a spatially-resolved 3′ mRNA library from biological tissue sections. This enables a "tissue-to-data" solution through in situ capture of the whole transcriptome at nanoscale resolution and centimeter-sized Field of View (FOV). This kit uses DNB-patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ.
- Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.
Application
- This novel tool explores spatial biology with unprecedented field-of-view, and subcellular resolution, enabling simultaneous transcriptome study and analysis at tissue-, cellular-, subcellular- and molecular level.
- Compatible with fresh frozen samples, and has reliable performance on cells, organs, and embryo sections of various animal and plant species..
STOmics Solutions
STEREO-SEQ TRANSCRIPTOMICS SOLUTION
• This enables a “tissue-to-data” experience through in situ capturing of the whole transcriptome at nanoscale resolution and centimeter-sized field of view.
Product Highlights
• Compatible with variety of tissues types & species as well as precious samples with low RIN values
• Improved capture efficiency giving higher gene counts per cell
STEREO-SEQ TRANSCRIPTOMICS SET FOR MIF
• This allows for in-depth spatial multi-omics discovery & also enables spatial visualization of multiple proteins in single-cell resolution on top of the unbiased whole transcriptome information on the same tissue section.
Product Highlights
• High fidelity protein staining imaging for enhanced spatial protein detection
• Detect multiple proteins with unbiased whole transcriptomics & flexibility in choosing antibodies based on user’s need.
• Co-detection of protein and RNA in a single experiment for extensible applications in tissue and cellular type study..
STEREO-SEQ LARGE CHIP DESIGNS
• This achieves both centimeter level FOV (now up to 2cm x 3cm) with subcellular resolution for efficient capturing of the whole transcriptome with true nanoscale resolution.
Product Highlights
• Compatible for all species.
• Large capture area without compromising the capture efficiency
• Proprietary cell segmentation algorithm integrated on Stereo-seq Analysis Workflow converting data to information
STEREO-CITE PROTEO-TRANSCRIPTOMICS SOLUTION
• This explores the study of tissue heterogeneity and associated biological significance in disease tissues, leading to a broader understanding of spatial omics studies with true single-cell resolution for both transcriptome & protein analysis
Product Highlights
• Simultaneous detection of the whole transcriptome and high detection efficiency of 100+ plex protein antibody combinations of your choice on the same tissue section.
• No auto-fluorescence interference or antigen instability caused by multiple rounds of detection.
STEREO-SEQ OMNI FOR FFPE SOLUTION
• This spatial transcriptomics solution helps to study your FFPE samples. It provides true spatial single-cell total RNA information with accuracy and precision with the innovative 'Random Probe' design.
Product Highlights
• Compatible with low-quality samples
• True single-cell level gene expression profiling combined with histology study
• Species agnostic, enabling study of host interaction with spatial context
• Random probe design for efficient total RNA capturing.
STOMICS Products & workflow
STOmics Products
Overall Components:
- Stereo-seq Permeabilization kit
- Stereo-seq Chip P (1cm * 1cm) (1 cm *2 cm), (2 cm *2 cm), (2 cm *3 cm)
- STOmics Accessory Kit
- Stereo-seq Transcriptomics T Kit
- Stereo-seq Chip T Slide (1cm * 1cm)
- Stereo-seq Chip N Slide (1cm * 1cm)
- Stereo-seq Protein Assisted Kit
- Stereo-seq16 Barcode Library Preparation Kit V1.0
- Stereo-seq PCR Adaptor
- STOmics FFPE Accessory Kit
- Stereo-seq Transcriptomics N Kit
- Stereo-seq Library Preparation kit
PROTOCOL
- Sectioning & mounting of fresh frozen samples on Stereo -seq Chip T Slide
- Stain the tissue with nuclei-or hematoxylin and eosin (H&E) stain
- Visualize it using an epi-fluorescence microscope or reflected brightfield microscope
- Tissue permeabilization and mRNA
- Released mRNA is captured by spatially barcoded probes on Stereo-seq Chips
- Sequence on Stereo-seq Transcriptome FF library on DNBSEQ-G400 or DNBSEQ-T7
- Stereo-seq Analysis Workflow (SAW) to process your spatial gene expression data